There is a forum post which describes how to write both the DFU and USB code using ISP, but I was not able to make it work.Expression vectors: how to choose or customize vectors for gene & protein expression Rachel Speer, Ph.D. To restore the DFU loader, use (1) or see README.txt in $SRC/hardware/arduino/firmwares/atmegaxxu2 for how to upload UNO-dfu_and_usbserial_combined.hex. Since fiddling around to put the UNO in DFU mode looks spastic, I prefer (2). DFU programming will be disabled until the DFU code is re-flashed.
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